Nature study identifies how PRIMPOL helps restart stalled DNA replication
A Nature study reported that the enzyme PRIMPOL is recruited to stalled DNA replication sites through an interaction with the cell's replicative helicase.
Researchers used single-molecule imaging to examine how cells restart DNA replication after it stalls. They reported that PRIMPOL is brought to stalled replication sites by an interaction with the CMG helicase complex.
The study found that this process enables another enzyme, polymerase epsilon, to extend the newly made primer while limiting error-prone DNA synthesis.